primary antibodies for alpha-fetoprotein (afp)  (Elabscience Biotechnology)

Journal: Scientific Reports

Article Title: Wharton’s jelly-derived mesenchymal stem cells combined with praziquantel as a potential therapy for Schistosoma mansoni -induced liver fibrosis

doi: 10.1038/srep21005

Figure Lengend Snippet: Photomicrographs of immunohistochemical staining (immunostain, DAB, X400) of ( A ) human alpha fetoprotein (AFP), ( B ) cytokeratin-18 (CK-18), ( C ) Hep par-1, ( D ) alpha smooth muscle actin (α-SMA), and ( E ) vimentin in liver sections of control and treated groups. No expression was observed in infected control and praziquantel (PZQ)-treated groups, whereas newly-formed liver-like cells in groups treated with Wharton’s jelly-derived mesenchymal stem cells (WJMSCs) showed positive expression (brown cytoplasmic discolouration) for AFP, CK-18, and Hep par-1. Positive expression of α-SMA and vimentin was observed only in scattered liver-like cells in groups treated with WJMSCs with the least expression observed in the groups which received WJMSCs combined with PZQ.

Article Snippet: Immunohistochemistry was performed by using an avidin-biotin complex immunoperoxidase technique with anti-human primary antibodies against alpha fetoprotein (AFP) (Cat. No. sc-51506, Santa Cruz Biotechnology, CA, USA) cytokeratin (CK)-7 (Cat. No. sc-17116, Santa Cruz Biotechnology, CA, USA), CK-18 (Cat. No. sc-6259, Santa Cruz Biotechnology, CA, USA), Hep par-1 (Cat. No. sc-58693, Santa Cruz Biotechnology, CA, USA), oval cell marker type 6 (OV-6) (Cat. No. MAB2020, R&D Systems, MN, USA), alpha smooth muscle actin (α-SMA) (Cat. No. M0851, Dako, CA, USA), and vimentin (Cat. No. sc-53464, Santa Cruz Biotechnology, CA, USA) diluted at 1:100, in PBS.

Techniques: Immunohistochemical staining, Staining, Control, Expressing, Infection, Derivative Assay

Journal: Scientific Reports

Article Title: Wharton’s jelly-derived mesenchymal stem cells combined with praziquantel as a potential therapy for Schistosoma mansoni -induced liver fibrosis

doi: 10.1038/srep21005

Figure Lengend Snippet: Effects of treatment with Wharton’s jelly-derived mesenchymal stem cells (WJMSCs), given either alone or combined with praziquantel (PZQ), on the immunohistochemical expression of human ( A ) alpha fetoprotein (AFP), ( B ) cytokeratin-7 (CK-7), ( C ) CK-18, ( D ) Hep par-1, ( E ) oval cell marker type-6 (OV-6), ( F ) alpha smooth muscle actin (α-SMA), and ( G ) vimentin in the liver sections of mice infected with S. mansoni . WJMSCs (1.5 × 10 6 cells/mouse) were injected at either the 8 th (early) or 16 th (late) week (W) post infection. PZQ (500 mg/kg/day) was orally given at the 7 th W post infection for 2 consecutive days. Animals were sacrificed at the 10 th month post infection. Values are presented as means ± S.E. (n = 8–10). Significantly different (P < 0.05) £ versus WJMSCs (8W), @ versus WJMSCs (16W), and † versus PZQ + WJMSCs (8W). Statistical analysis was performed by one-way analysis of variance (One-way ANOVA) followed by Bonferroni post hoc test. O Significant interaction when PZQ and WJMSCs were combined using factorial design.

Article Snippet: Immunohistochemistry was performed by using an avidin-biotin complex immunoperoxidase technique with anti-human primary antibodies against alpha fetoprotein (AFP) (Cat. No. sc-51506, Santa Cruz Biotechnology, CA, USA) cytokeratin (CK)-7 (Cat. No. sc-17116, Santa Cruz Biotechnology, CA, USA), CK-18 (Cat. No. sc-6259, Santa Cruz Biotechnology, CA, USA), Hep par-1 (Cat. No. sc-58693, Santa Cruz Biotechnology, CA, USA), oval cell marker type 6 (OV-6) (Cat. No. MAB2020, R&D Systems, MN, USA), alpha smooth muscle actin (α-SMA) (Cat. No. M0851, Dako, CA, USA), and vimentin (Cat. No. sc-53464, Santa Cruz Biotechnology, CA, USA) diluted at 1:100, in PBS.

Techniques: Derivative Assay, Immunohistochemical staining, Expressing, Marker, Infection, Injection

Journal: Scientific Reports

Article Title: Wharton’s jelly-derived mesenchymal stem cells combined with praziquantel as a potential therapy for Schistosoma mansoni -induced liver fibrosis

doi: 10.1038/srep21005

Figure Lengend Snippet: Effects of treatment with Wharton’s jelly-derived mesenchymal stem cells (WJMSCs), given either alone or combined with praziquantel (PZQ), on the gene expression of human ( A ) albumin (Alb) and ( B ) alpha fetoprotein (AFP) and murine ( C ) alpha smooth muscle actin (α-SMA), ( D ) collagen-I (Col-I), and ( E ) interleukin-13 (IL-13) in the liver tissue of mice infected with S. mansoni . WJMSCs (1.5 × 10 6 cells/mouse) were injected at either the 8 th (early) or 16 th (late) week (W) post infection. PZQ (500 mg/kg/day) was orally given at the 7 th W post infection for 2 consecutive days. Animals were sacrificed at the 10 th month post infection. Values are presented as means ± S.E. (n = 8-10). Significantly different (P < 0.05) * vs infected control, $ vs PZQ, £ vs WJMSCs (8W), @ vs WJMSCs (16W), and † vs PZQ + WJMSCs (8W). Statistical analysis was performed by one-way analysis of variance (One-way ANOVA) followed by Bonferroni post hoc test. O Significant interaction when PZQ and WJMSCs were combined using factorial design.

Article Snippet: Immunohistochemistry was performed by using an avidin-biotin complex immunoperoxidase technique with anti-human primary antibodies against alpha fetoprotein (AFP) (Cat. No. sc-51506, Santa Cruz Biotechnology, CA, USA) cytokeratin (CK)-7 (Cat. No. sc-17116, Santa Cruz Biotechnology, CA, USA), CK-18 (Cat. No. sc-6259, Santa Cruz Biotechnology, CA, USA), Hep par-1 (Cat. No. sc-58693, Santa Cruz Biotechnology, CA, USA), oval cell marker type 6 (OV-6) (Cat. No. MAB2020, R&D Systems, MN, USA), alpha smooth muscle actin (α-SMA) (Cat. No. M0851, Dako, CA, USA), and vimentin (Cat. No. sc-53464, Santa Cruz Biotechnology, CA, USA) diluted at 1:100, in PBS.

Techniques: Derivative Assay, Gene Expression, Infection, Injection, Control

Journal: PLoS ONE

Article Title: Enhanced Hepatogenic Transdifferentiation of Human Adipose Tissue Mesenchymal Stem Cells by Gene Engineering with Oct4 and Sox2

doi: 10.1371/journal.pone.0108874

Figure Lengend Snippet: (A) The mRNA expression level of albumin (ALB) was strongly expressed in hepatogenically differentiated Oct4/Sox2-ATMSCs, whereas the expression level of α-fetoprotein (AFP) was lower than that of RFP-ATMSCs. The expression levels of transferrin were not significantly different in both cells. Undifferentiated ATMSCs and HepG2 were used as negative and positive controls, respectively. (B) Hepatocyte-like cells from RFP- and Oct4/Sox2-ATMSCs are confirmed by immunofluorescence staining for AFP and ALB. Nuclei were counterstained with Hoecst33342.

Article Snippet: The cells were incubated overnight sequentially at 4°C with primary antibodies raised against α-fetoprotein [(AFP) 1:50; goat, Santa Cruz] and albumin [(ALB) 1:50; mouse, Santa Cruz].

Techniques: Expressing, Immunofluorescence, Staining